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Construction of a complete set of Neisseria meningitidis defined mutants - the NeMeSys collection - and its use for the phenotypic profiling of the genome of an important human pathogen
[article]
2020
bioRxiv
pre-print
One of the most important challenges in biology is to determine the function of millions of genes of unknown function. Even in model bacterial species, there is a sizeable proportion of such genes, which has important theoretical and practical consequences. Here, we constructed a complete collection of defined mutants - named NeMeSys - in the important human pathogen Neisseria meningitidis, consisting of individual mutants in 1,584 non-essential genes. This effort identified 391 essential
doi:10.1101/2020.08.12.247981
fatcat:oapiryjhkngmhakm7jpoj442rq