Characteristic Interactions with Phosphatidylinositol 4,5-Bisphosphate Determine Regulation of Kir Channels by Diverse Modulators

Xiaona Du, Hailin Zhang, Coeli Lopes, Tooraj Mirshahi, Tibor Rohacs, Diomedes E. Logothetis
2004 Journal of Biological Chemistry  
The activity of specific inwardly rectifying potassium (Kir) channels is regulated by any of a number of different modulators, such as protein kinase C, G q -coupled receptor stimulation, pH, intracellular Mg 2؉ or the ␤␥subunits of G proteins. Phosphatidylinositol 4,5bisphosphate (PIP 2 ) is an essential factor for maintenance of the activity of all Kir channels. Here, we demonstrate that the strength of channel-PIP 2 interactions determines the sensitivity of Kir channels to regulation by the
more » ... various modulators. Furthermore, our results suggest that differences among Kir channels in their specific regulation by a given modulator may reflect differences in their apparent affinity of interactions with PIP 2 .
doi:10.1074/jbc.m403413200 pmid:15155739 fatcat:kobed7cfbbg55i63be2alnhunu