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The effect of amniotic membrane stem cells as donor nucleus on gene expression in reconstructed bovine oocytes
International Journal of Developmental Biology
Nuclear reprogramming of a differentiated cell in somatic cell nuclear transfer (SCNT) is a major concern in cloning procedures. Indeed, the nucleus of the donor cell often fails to express the genes which are a prerequisite for normal early embryo development. This study was aimed to evaluate the developmental competence and the expression pattern of some reprogramming related genes in bovine cloned embryos reconstructed with amniotic membrane stem cells (AMSCs) in comparison with thosedoi:10.1387/ijdb.160010hn pmid:27389982 fatcat:mfrgqw53ibfxjgj3pcr35ewvfe