ANTIHYPERLIPIDEMIC AND ANTIOXIDANT ACTIVITY OF BIOFRACTIONS OF FLOWERS OF HIBISCUS ROSA-SINENSIS IN POLOXAMER 407 INDUCED HYPERLIPIDEMIC RATS

Mehul G. Rana
2017 World Journal of Pharmacy and Pharmaceutical Sciences  
Hyperlipidemia is considered to be one of the greatest risk factors contributing to the prevalence and severity of cardiovascular diseases. Hyperlipidemia was induced by poloxamer 407 (30% w/w in distilled cool water; 500 mg/kg, i.p). Prior to the administration of P-407, the rat of reference group were administered with atorvastatin (50 mg/kg p.o.) and finofibrate (FF 65 mg/kg p.o.) while test groups received 1 hr before dose (100 mg/kg p.o and 200 mg/kg p.o of each bio-fractions) of various
more » ... tions) of various bio-fractions (Benzene, Ethyl acetate, Methanol, Water) of methanolic extract of plant. Serum lipid profiles were investigated after 3, 6 and 24 hour of treatment. The result were found that ethyl acetate fractions of methanolic extract significantly reduced the serum levels of total cholesterol (TC) (p < 0.05), triglycerides (TG) (p < 0.001) and very low density lipoprotein (VLDL) (p < 0.001) as compare to the disease control rats after 6 h of treatment. After 24 h of treatment, the ethyl acetate fractions of extract induced a significant reduction in serum TC (p < 0.001), TG (p < 0.001), VLDL (p < 0.001), low density lipoprotein (LDL) (p < 0.05) and significant increase in high density lipoprotein ( HDL) levels (p < 0.001), the benzene fractions of extract induced a significant reduction in serum TC (p < 0.05), TG (p < 0.01), VLDL (p < 0.01) and not significantly reduced LDL (p < 0.05) and not significant increase in HDL levels, when compared to disease control group. All these effects were comparable to those of the reference standard, atorvastatin and finofibrates. Mehul et al. World Journal of Pharmacy and Pharmaceutical Sciences which is claimed to possess antioxidant, antihyperlipidemic activities suggesting its usefulness in the treatment of hyperlipidemia may pave way for newer and better therapies. Hibiscus rosasinensis Linn. (Family Malvaceae), is widely distributed throughout the world. As a traditional medicine, the fresh juice of flower of wild variety is used to treat gonorrhaea, the powdered root is used to treat menorrhagia and the infusion of the petals is used as refrigerant drink in fevers. [11] The alcoholic extract of flowers of H. sabdariffa inhibited angiotensin-I converting enzymes. [12] The alcoholic extract of flowers of H. rosasinesis has been proved to posseses anticonvulsant property. [13] All the parts of Hibuscus rosa-sinensis L and chem. constituents are used as lowering of blood pressure [14] , antiacne [15] , antipyretic and analgesic activity [16] , hypotensive effect in deoxycorticosterone acetate (DOCA)-salt and fructose-induced hypertensive rats. [17] [18] [19] [20] [21] The principal constituents of Hibiscus rosa sinensis Linn. are flvones. Flvones contain quercetin-3-diglucoside, quercetin-3,7-diglucoside, cyaniding-3,5-diglucoside, quercetin-3sophorotrioside, kaempferol-3xylosylglucoside, cyaniding-3-sophoroside-5-glucoside and other constituent are cyclopeptide alkaloid, cyanidin chloride, hentriacontane, riboflavin, ascorbic acid, thiamine, taraxeryl acetate, β-sitosterol, cyclicacids sterculic and malvalic acids. [22]
doi:10.20959/wjpps20177-9618 fatcat:xsxmai6x7vdmlaxprvw6cyx25i