In vitro Quantitative Kinetic Study of Infectious Pancreatic Necrosis Viral Antigen by Flow Cytometry

Sylvia Rodríguez, M. Pilar Vilas, Carmen Gutierrez, Sara I. Pérez
1995 Gyobyo kenkyu - Fish pathology  
Infectious pancreatic necrosis virus (IPNV) was infected in CHSE-214 cells, and viral antigens in the cells stained with indirect immunofluorescence method were monitored by flow cytometer during early multiplication stage of the virus. Viral antigen could first be detected at 2 h post infection (p.i.), and the larger numbers of labeled CHSE-214 cells were obtained at 8 and 24 h p.i. in both VR-299 and Sp strains of IPNV examined. The detection method is fast and sensitive and yields
more » ... d yields quantitative results on single cell basis.
doi:10.3147/jsfp.30.1 fatcat:od6hkloprzgv3gfliiag57y6im