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Photorespiration consumes fixed carbon and energy generated from photosynthesis to recycle glycolate and dissipate excess energy. The aim of this study was to investigate whether we can use the energy that is otherwise consumed by photorespiration to improve the production of chemicals which requires energy input. To this end, we designed and introduced an isoprene synthetic pathway, which requires ATP and NADPH input, into the cyanobacterium Synechocystis sp. 6803. We then deleted the glcD1doi:10.1186/s40643-021-00398-y doaj:8c58836911c64c2986fee3541c4549b4 fatcat:3w64c6aw2fflzij2ncqnqhzi4u