In vitro regeneration of Pakistani peanut (Arachis hypogea L.) varieties using de-embryonated coteledonary explants

Nazir Farhat, Mahmood ul Hassan, Akram Zahid, Matloob Javed Muhammad, Ali Shoukat, Muhammad Ali Ghulam, Zafar Yusuf
2011 African Journal of Biotechnology  
Peanut (Arachis hypogaea L.) belongs to the family leguminosae and is one of the world's largest oilseed crops. This study is the first report on peanut regeneration from Pakistan using four commercially released peanut varieties, that is, Golden, BARI-2000, BARD-479 and BARD-92. Longitudinally, halved cotyledons with removed embryos were employed as explants. Among various tested combinations of BAP and NAA, the best combination was 4 and 0.1 mg/L respectively. BARI-2000 proved to be the best
more » ... esponsive variety for in vitro regeneration in terms of number of shoots/explant (133.3%) and number of rooted plants/explant (124.99%). The optimized protocol, which is the first one in the country, would be used to incorporate important traits in future breeding programmes of peanut in Pakistan.
doi:10.5897/ajb11.381 fatcat:wq65d5pzlzhhhfqjykrtfafi4q