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The impact of different physicochemical parameters of fermentation on extracellular cellulolytic enzyme production by Trichoderma harzianum
Journal of Crop Protection
This study was undertaken to find out the optimum physicochemical parameters of fermentation, i.e. pH, incubation temperature and incubation time for the cellulase enzyme production of Trichoderma harzianum. The extracellular protein content was estimated by the dye binding method of Bradford. Endo-glucanase (EG), exoglucanase (or Cellobiohydrolase; CBH), β-glucosidase and total cellulase activity were investigated. The molecular weight of cellulase enzymes was studied using SDS-PAGE. Tofatcat:ndcspberzna3lo6akylkqe2plq