Quantitative Glycomics Strategies

Yehia Mechref, Yunli Hu, Janie L. Desantos-Garcia, Ahmed Hussein, Haixu Tang
2013 Molecular & Cellular Proteomics  
The correlations between protein glycosylation and many biological processes and diseases are increasing the demand for quantitative glycomics strategies enabling sensitive monitoring of changes in the abundance and structure of glycans. This is currently attained through multiple strategies employing several analytical techniques such as capillary electrophoresis, liquid chromatography, and mass spectrometry. The detection and quantification of glycans often involve labeling with ionic and/or
more » ... ydrophobic reagents. This step is needed in order to enhance detection in spectroscopic and mass spectrometric measurements. Recently, labeling with stable isotopic reagents has also been presented as a very viable strategy enabling relative quantitation. The different strategies available for reliable and sensitive quantitative glycomics are herein described and discussed. Molecular & Cellular Proteomics
doi:10.1074/mcp.r112.026310 pmid:23325767 pmcid:PMC3617334 fatcat:5azffaqxgneophhygbp4uka6fe