32P-labeled TMV was inoculated onto tobacco leaves . At times after inoculation, the leaves were homogenized and the homogenate was fractionated into precipitates of 600g centrifugation (P1), of 15,000g centrifugation (P2), of 105,000g centrifugation (P3), and acid-insoluble (P4), acid-soluble (S) fractions of the supernatant. The radioactivity in P3 fraction increased and that in P1 fraction decreased until 6 hours after inoculation, then the former decreased while the latter increased. RNase

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... reatment of the homogenate showed the same radioactive distribution among fractions as that in nontreatment. The radioactivity in S fraction, though the amount was small, was higher during 6 hours after inoculation than that of later stages. The fate of TMV immediately after the introduction into host cells is discussed.