Possibility of Use of Agricultural Byproducts for Mass Production of Beauveria bassiana (Balsamo) Vuillemin to Control Coffee Berry Borer (Hypothenemus hampi (Ferrari))

M. R. V. N. Subasinghe, K. G. A. P. K. Amarasinghe, M. Dharmadasa
2013 Journal of Food and Agriculture  
Coffee Berry Borer (Hypothenemus hampei (Ferrari)) is the most frequently occurring and destructive pest of coffee (Coffea spp.) in Sri Lanka. Beauveria bassiana (Balsamo) Vuellemin is an entomopathogenic fungus that has a great potential as a biological control agent for the control of coffee berry borer. An experiment was carried out to investigate the effect of different agricultural byproducts including five solid substrates i.e. coir dust, saw dust, refused tea, disposable parts of maize
more » ... b (pith + chaff + woody ring) and oil cake, and two liquid substrates i.e. coconut water and molasses. Mass production was done under two temperature levels, at room temperature (30 ± 2 0 C) under incubator temperature (25 ± 2 0 C). Fungus was inoculated into sterilized solid and liquid substrates in equal concentrations. Inoculated substrates were placed in the incubator and under room temperature and experiment continued for 8 weeks. The highest number of spores were observed in molasses (6.9475x10 13 spores/ml) among all the tested substrates. There was no significant difference (p > 0.05) with respect to the spore production in all substrates at room and incubator temperatures. Optimum spore production was achieved 42 days after inoculation of the fungus in all substrates irrespective of the temperature.
doi:10.4038/jfa.v6i1-2.5185 fatcat:mxnysg7lfbhwtprqsstys62iea