The heamatological profile of two freshwater fishes Labeo rohita (Rohu) and Channa striatus (Murrel) with a relationship to the sex and the water quality under natural habitat was analyzed. The freshwater fishes were collected from the pond located in Thodaneri, Vadipatti taluk, Madurai district, Tamil Nadu, India. In heamatology studies, no significant variation was observed in Heamoglobin content and found almost the same in both the fish irrespective of the sex and also falls in the range of

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... control value. In differential Leucocyte count (DLC) the following blood components Lymphocyte, Eosinophil, and Monocyte count were significantly higher than the control values and the values are found maximum in Rohu when compared to Murrel.RBC (Erythrocyte) count was almost the same in both the fish and the value falls between the range of control value Packed Cell Volume (PCV) count was almost the same in both the fish and the value falls between the range of control value. The values of Hb and PCV were found higher in male fish as compared to female fish. The leucocyte count is also higher in the males when compared to the females of both Rohu and Murrel. These may be also influenced by physiological activity, feeding habits, infectious agents, water quality parameters, and the environmental stress on the organism.