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Recebido em 1/2/02; aceito em 14/2/03 2,5-HEXANODIONE DETERMINATION IN URINE SAMPLES SUBJECTED TO ACID HYDROLYSIS: RELEVANCE OF THE CHROMATOGRAPHIC COLUMN AND THE pH. Acidic hydrolysis of samples is frequent in urinary 2,5-hexanodione determination. This hydrolysis should be performed under proper conditions, in order to avoid interference, such as the presence of 2,5-dimethylfurane and 2-acetylfurane. The results of the present work, as well as data from the literature, show that the use ofdoi:10.1590/s0100-40422003000400021 fatcat:5xcdbekebbblviqqthjjwy6s2a