Investigation of Halohydrins Degradation by Whole Cells and Cell-free Extract of Pseudomonas putida DSM 437: A Kinetic Approach

A. Konti
2017 Chemical and Biochemical Engineering Quarterly  
The biodegradation of two halohydrins (1,3-dichloro-2-propanol and 3-chloro-1,2propanediol) by P. putida DSM 437 was investigated. Intact cells of previously acclimatized P. putida DSM 437 as well as cell-free extracts were used in order to study the degradation kinetics. When whole cells were used, a maximum biodegradation rate of 3-CPD (v max = 1.28 . 10 -5 mmol mg -1 DCW h -1 ) was determined, which was more than 4 times higher than that of 1,3-DCP. However, the affinity towards both
more » ... owards both halohydrins (K m ) was practically the same. When using cell-free extract, the apparent v max and K m values for 1,3-DCP were estimated at 9.61 . 10 -6 mmol mg -1 protein h -1 and 8.00 mM, respectively, while for 3-CPD the corresponding values were 2.42 . 10 -5 mmol mg -1 protein h -1 and 9.07 mM. GC-MS analysis of cell-free extracts samples spiked with 1,3-DCP revealed the presence of 3-CPD and glycerol, intermediates of 1,3-DCP degradation pathway. 3-CPD degradation was strongly inhibited by the presence of epichlorohydrin and to a lesser extent by glycidol, intermediates of dehalogenation pathway.
doi:10.15255/cabeq.2016.924 fatcat:6hsc4yrdgrdbdaxbeyjhddenum