injected intravenously every four or five days four or five times with increasing quantities of urine containing Bence-Jones protein. The amounts injected have been 2, 4, 8, 12 and 16 or 20 c.c. as a rule, and the animals have been bled on the seventh or eighth day after the last injection because at that time the antibody content of the serum usually has reached its high point. The tests are made in small tubes by the contact or ring method, a small quantity of antiserum being placed below the

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... dilution of urine or blood serum, and the results are read after one hour at the room temperature.