Selective induction of an embryonic fibronectin isoform in the rat aorta in vitro

M Hosoi, I Takasaki, A Pavlova-Rezakova, H Himeno, A V Chobanian, P Brecher
1993 Circulation Research  
thesis and steady-state mRNA levels increased.' In the present study, we used rat aortic rings to characterize further the changes in fibronectin expression during in vitro incubation and also compared the changes in fibronectin to those of other extracellular matrix genes. Materials and Methods Materials Male Wistar rats, 250 to 300 g, were obtained from Charles River Breeding Laboratories, Wilmington, Mass. Dulbecco's modified Eagle medium (DMEM) and genistein were purchased from GIBCO
more » ... d from GIBCO Laboratories, Grand Island, NY. Gene Screen Plus nylon membrane, [a-32P]dCTP, [a-32P]CTP, [a-"5S]UTP, Econoflour, and Protosol were obtained from DuPont/New England Nuclear Corp, Boston, Mass. A multiprime labeling kit and RNA ladders were purchased from Amersham Corp, Arlington Heights, Ill. RNase A, RNase Ti, protein K, and in vitro transcription kits were purchased from Ambion, Inc, Austin, Tex. Cycloheximide, polymyxin B sulfate, paraformaldehyde, and dimethyl sulfoxide were purchased from Sigma Chemical Co, St Louis, Mo. T7 and SP6 RNA polymerases were from Promega Corp, Madison, Wis.
doi:10.1161/01.res.73.4.689 pmid:8370123 fatcat:gont3fcek5e5vhrkpunlo6v5km