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Estimating Bacterial and Cellular Load in FCFM Imaging
2018
Journal of Imaging
We address the task of estimating bacterial and cellular load in the human distal lung with fibered confocal fluorescence microscopy (FCFM). In pulmonary FCFM some cells can display autofluorescence, and they appear as disc like objects in the FCFM images, whereas bacteria, although not autofluorescent, appear as bright blinking dots when exposed to a targeted smartprobe. Estimating bacterial and cellular load becomes a challenging task due to the presence of background from autofluorescent
doi:10.3390/jimaging4010011
fatcat:nt6arqviwfd3xixqdxo2ktm4ki