Additional file 5. Figure S5. 3D visualization of a dorsally emerged partial cilia. A XY, XZ and YZ projections of Hoechst (blue), pH Smo (green), anti-GFP (red), and acetylated tubulin, Ac Tub (purple) staining of a ventrally emerged outside cilium imaged by SDCM. Dashed line marks the cell outline. D Dorsal cell surface, V Ventral cell surface. Merged image is of Hoechst, pH Smo and the anti-GFP signal. Closed arrowhead indicates ciliary staining. B Z planes of the merged image of Hoechst, pH

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... Smo and the anti-GFP signal of the same cell. Arrow indicates the emerged cilia tip. VENTRAL indicates the ventral anti-GFP surface staining, while DORSAL indicates the dorsal anti-GFP staining.