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The nucleotide composition of the 5′UTR of the yellow fever virus (YFV) has been reported to be the most constant sequence in the viral genome, but a comprehensive analysis of this constancy has not been presented. The current report is an analysis of the 5′UTRs from 48 sequences deposited in GenBank representing the seven described genotypes, five in Africa and two in the Americas. The YFV 5′UTRs consist of 118-120 nucleotides, 92% (110/119) of which are constant in all sequences. Thedoi:10.1101/2020.07.17.207902 fatcat:mom4fydac5dvhe4qijk32tjnu4