Acute Exacerbation of Chronic Obstructive Pulmonary Disease: Phenotypic Screening and Sensitivity of Microbiological Profile

Huma Firdaus, Nafees Ahmad Khan, Nazish Fatima, Mohammad Shameem, Rakesh Bhargava, Zuber Ahmad
2020 Journal of Clinical and Diagnostic Research  
A total of 200 patients admitted with the diagnosis of AECOPD were selected as study group. All the patients in the study group were more than 40 years of age. Inclusion criteria: Patients (previously and currently diagnosed COPD) were selected as per GOLD guidelines [11] and Anthonisen criteria for exacerbation of COPD [8]. Exclusion criteria: Patients of COPD having bronchiectasis, sputum positive tuberculosis, lung malignancy and other evident disease on chest X-ray (pneumothorax,
more » ... othorax, hydro-pneumothorax, pleural effusion, lung mass, lung abscess) were excluded. Patients with oral candidiasis were also excluded from the study. All the sputum samples were collected under strict aseptic precautions in sterile containers, properly labeled and were transported to the laboratory in appropriate conditions and processed according to standard guidelines [12]. Bartlett grading system was used for microscopic analysis of sputum samples [13]. To each sputum sample 2% (w/v) N-acetyl-L cysteine and a few glass beads (2.5-3.5 mm) were added. A pH of 7.3 was adjusted. Homogenised samples were diluted in brain heart infusion broth and plated on blood agar, chocolate agar and MacConkey agar as following:
doi:10.7860/jcdr/2020/44057.13804 fatcat:oypymhcaubfsxfokwqxniixrty