Oligomerization processes limit photoactivation and recovery of the Orange Carotenoid Protein [article]

Elena A. Andreeva, Stanislaw Nizinski, Adjele Wilson, Matteo Levantino, Elke De Zitter, Rory Munro, Fernando Muzzopappa, Aurelien Thureau, Ninon Zala, Gotard Burdzinski, Michel Sliwa, Diana Kirilovsky (+2 others)
2022 bioRxiv   pre-print
The Orange Carotenoid Protein (OCP) is a photoactive protein involved in cyanobacterial photoprotection, by quenching of the excess of light harvested energy. The photoactivation mechanism remains elusive, in part due to absence of data pertaining to the timescales over which structural changes in the protein take place. It also remains unclear whether or not oligomerization of the dark-adapted and light-adapted OCP could play a role in the regulation of its energy quenching activity. Here, we
more » ... robed photo-induced structural changes in OCP by a combination of static and time-resolved X-ray scattering and steady-state and transient optical spectroscopy in the visible range. Our results suggest that oligomerization partakes in the regulation of OCP photocycle, with different oligomers slowing down the overall thermal recovery of the dark-adapted state of OCP. They furthermore reveal that upon non-photoproductive excitation, a numbed-state forms, which remains in a non-photoexcitable structural state for at least ~0.5 microsecond after absorption of a first photon.
doi:10.1101/2022.02.04.479168 fatcat:2gipexxwtjc7rabuxyvwim53qe