Ninhydrin is the most well known spray reagent for identification of amino acids due to its high sensitivity. But, it produces same purple/violet color with all amino acids, except proline and hydroxy proline. A new spray reagent, para bromobenzoic acid has been introduced here which produces distinguishable colors with the amino acids with moderately high sensitivity (0.01-1.0) μg. A probable mechanism for such color formation has also been proposed.