Trends in genetic divergence of Biomphalaria alexandrina snails and Oreochromis niloticus fish in response to water quality of Lake Burullus, Egypt

Hanaa M. M. El-Khayat, Sohair Abd-Elkawy, Mervat A. Ahmed, Nouran A. Abou- Ouf, Wafaa A. Mohammed
2018 Egyptian Journal of Aquatic Biology and Fisheries  
Water quality of Lake Burullus was investigated through evaluating their impact on health and genetic alterations of native Lake Burullus Biomphalaria alexandrina snails and Oreochromis niloticus fish samples and non-native ones exposed in simulating experiments for six weeks to lake-water/sediment from three lake sites; El-Hamoul, Mottobus and Sidi-Salem. Water in El-Hamoul and Sidi-Salem lake sites and their simulating experiments showed higher levels of total dissolved solids and
more » ... while higher levels of Zn and Cd were recorded in their simulating experiments. Also, most of highest bioaccumulation levels in native and non-native samples were recorded in samples of either El-Hamoul or Sidi-Salem while Mottobus samples showed the least levels in most of water quality creiteria and bioaccumulation. Higher Cu bioaccumulation was observed in the native snails and in kidney of native Sidi-Salem fish samples while most non-native fish showed higher bioaccumulation in all organs. All snail hemolymph samples showed increase in granulocytes, decrease in hyalinocytes and total hemocytes while Hb decreased in native and slightly increased in non-native Sidi-Salem and El-Hamoul samples and vice versa in Mottobus. Fish blood showed highly significant increase in WBCs of native El-Hamoul and Sidi-Salem samples while samples of native and non-native Mottubus showed the highest Hb level. Genetic divergences were investigated by ISSR-PCR technique in snail and fish samples: native lake, non-native non-exposed (control), and non-natives of El-Hamoul, Sidi-Salem & Mottobus experiments. Results of PCR demonstrated 33 and 26 amplified bands of which 30 % and 35% were polymorphic among snail and fish samples, respectively. Cluster analysis using dendrograms based on genetic similarity matrices showed cluster between control, lake, and nonnative Mottobus groups; the non-native El-Hamoul sample was the most divergent followed by the non-native Sidi-Salem sample; indicating that water quality in Lake Burullus exerted divergence among snail and fish genomic DNA graded from El-Hamoul > Sidi-Salem > Mottobus samples.
doi:10.21608/ejabf.2018.11113 fatcat:k2dkrxvmn5hipaudgxeyjadlom