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Heterologous Expression and Purification of SARS-CoV2 Nucleocapsid Protein
2020
Bio-protocol
Preserved Fulltext
This protocol describes a step by step method for heterologous expression of SARS-CoV2 Nucleocapsid (N) protein in Escherichia coli. Moreover, this protocol includes steps to purify the N protein to high purity and homogeneity. Thus, purified protein can be used for ligand binding assays and other biochemical experiments.
doi:10.21769/bioprotoc.3720
fatcat:ibqdeddvlzgs3f3yxqb5g3y7ay