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Demonstration by Freeze-Etching of a Single Cleavage Plane in the Cell Wall of a Gram-Negative Bacterium
Journal of Bacteriology
In the examination of protoplasts of a gram-negative bacterium classified as a Pseudomonas sp. by freeze-etching, we found a smooth external surface which is not seen if the preparations are not "etched." This external structure is seen as a sleeve surrounding and connecting the cells in unetched preparations, and we present evidence that it is a eutectic formed during the freezing of the specimen. In the system used in this study, the four layers of the cell wall of a gram-negative bacteriumdoi:10.1128/jb.106.2.659-671.1971 fatcat:xarkpeswcbeovlmz4nns4dhxl4