An automated workflow for the discovery and docking simulation of the protein-protein complexes using in vivo chemical cross-linking [article]

Vladimir Svetlov, Evgeny Nudler
2018 bioRxiv   pre-print
We report an automated/score driven workflow of discovery and structural interrogation of protein-protein complexes in vivo by means of chemical cross-linking. This streamlined workflow is illustrated by the discovery of the direct interactions in vivo between E. coli RNAP polymerase and the transcription elongation factor NusA, using chemical cross-linking as the proximity sensor, and the generation of a near-native structure of NusA-NTD/RNA polymerase complex by distance restraints-guided protein-protein docking.
doi:10.1101/373829 fatcat:iytn5ansszgntcnqld64vccun4