The present study was conducted to determine antibacterial efficacy of actinomycetes isolated from rhizosphere soils of Shikaripura, Karnataka, India. A total of 32 actinomycetes were recovered on Starch casein nitrate agar. Primary screening by cross streak method revealed marked antibacterial activity in case of three isolates viz., SRDP-S-03, SRDP-S-05 and SRDP-S-30. These isolates were grown in Starch casein nitrate broth and the culture filtrates were extracted using ethyl acetate.

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... y screening for antibacterial activity of ethyl acetate extract was performed by agar well diffusion assay. The extracts have shown marked inhibition of Gram positive bacteria when compared to Gram negative bacteria. Microscopic characteristics and other characteristics assisted identification of three actinomycete isolates as members of Streptomyces. Further studies on characterization of isolates and purification of bioactive compounds from ethyl acetate extracts are under progress.