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Proteomics and Phosphoproteomics Analysis of Human Lens Fiber Cell Membranes
2013
Investigative Ophthalmology and Visual Science
The human lens fiber cell insoluble membrane fraction contains important membrane proteins, cytoskeletal proteins, and cytosolic proteins that are strongly associated with the membrane. The purpose of this study was to characterize the lens fiber cell membrane proteome and phosphoproteome from human lenses. HPLC-mass spectrometry-based multidimensional protein identification technology (MudPIT), without or with phosphopeptide enrichment, was applied to study the proteome and phosphoproteome of
doi:10.1167/iovs.12-11168
pmid:23349431
pmcid:PMC3567755
fatcat:hmaqofsu5jgahefbpvwaoiq65u