3D Structure Studies of the Pancreatic Beta Cell by High Resolution Cellular Tomography

B. J. Marsh, D. N. Mastronarde, K. E. Howell, J. C. Hutton, C. J. Rhodes, J. R. McIntosh
2003 Microscopy and Microanalysis  
We have used high-pressure freezing, freeze-substitution and dual-axis electron microscope (EM) tomography to study the organelles involved in the synthesis, processing and secretion of insulin in three dimensions (3D) at ~6nm resolution in the pancreatic beta cell line HIT-T15. These data have: (1) revealed the complexity of structural relationships among the Golgi, endoplasmic reticulum (ER) and compartments of the endosomal-lysosomal system [1], (2) provided evidence that multiple transport
more » ... echanisms act in concert in the same region of the Golgi ribbon [2], and (3) suggested a role for the ER in regulating membrane traffic/sorting at the trans Golgi -the presumptive site where proinsulin is sorted and packaged into nascent secretory granules [1, 2] .
doi:10.1017/s1431927603445789 fatcat:5uotakqhojasncv2unoeh7lbzm