Nuclear Factor-κB Directs Carcinoembryonic Antigen-related Cellular Adhesion Molecule 1 Receptor Expression inNeisseria gonorrhoeae-infected Epithelial Cells

Petra Muenzner, Oliver Billker, Thomas F. Meyer, Michael Naumann
2001 Journal of Biological Chemistry  
The human-specific pathogen Neisseria gonorrhoeae expresses opacity-associated (Opa) protein adhesins that bind to various members of the carcinoembryonic antigen-related cellular adhesion molecule (CEACAM) family. In this study, we have analyzed the mechanism underlying N. gonorrhoeae-induced CEACAM up-regulation in epithelial cells. Epithelial cells represent the first barrier for the microbial pathogen. We therefore char- (NF-B) , which translocates as a p50/p65 heterodimer into the nucleus,
more » ... and an NF-B-specific inhibitory peptide inhibited CEACAM1-receptor up-regulation in N. gonorrhoeae-infected HOSE cells. Bacterial lipopolysaccharides did not induce NF-B and CEACAM up-regulation, which corresponds to our findings that HOSE cells do not express toll-like receptor 4. The ability of N. gonorrhoeae to up-regulate its epithelial receptor CEACAM1 through NF-B suggests an important mechanism allowing efficient bacterial colonization during the initial infection process. acterized CEACAM expression in primary human ovarian surface epithelial (HOSE) cells and found that CEACAM1-3 (L, S) and CEACAM1-4 (L, S) splice variants mediate an increased Opa 52 -dependent gonoccocal binding to HOSE cells. Up-regulation of these CEACAM molecules in HOSE cells is a direct process that takes place within 2 h postinfection and depends on close contact between microbial pathogen and HOSE cells. N. gonorrhoeae-triggered CEACAM1 up-regulation involves activation of the transcription factor nuclear factor B
doi:10.1074/jbc.m108135200 pmid:11751883 fatcat:jurifayxb5hatiblnj2fcvb2xi