Radiosensitizing potential of rutin against human colon adenocarcinoma HT-29 cells

M. Vijay, G. Sivagami, K. Thayalan, N. Nalini
2016 Bratislava Medical Journal  
AIM: The present study was focused at evaluating the potential of rutin to improve the radiotherapeutic index and thereby the cytotoxicity towards colon cancer cells. MATERIALS AND METHODS: HT-29 cells were pre-treated with rutin and the effect on cell proliferation was determined by MTT assay followed by exposure to radiation. After irradiation, experimental groups were sham control, rutin alone, radiation alone, rutin along with radiation-treated HT-29 cells. RESULTS: Cytotoxicity study
more » ... rated that treatment of HT-29 cells with different concentrations of rutin reduced cell proliferation in a dose-and time-dependent manner. After irradiation, the HT-29 cells revealed that the combined effect of 4 Gy radiation and rutin at 80 μM concentration showed a decrease in cell viability as compared to rutin-alone treated and 4 Gy-alone irradiated HT-29 cells. Furthermore, the increase in apoptotic cells, change from normal nuclei to abnormal nuclei, alterations in mitochondrial membrane potential, increase in DNA damage, increase in levels of lipid peroxidative markers, and decrease in antioxidant status were observed in 4 Gy-and rutin-treated group as compared to the other treated groups. CONCLUSIONS: Combined effect of rutin and radiation in HT-29 cells leads to a more pronounced cell death rate. Thus, rutin exhibits radiosensitizing effects on Fig. 8, Ref. 42). Text in PDF Acknowledgement: The authors gratefully acknowledge Dr. Kamakshi Memorial hospital, Chennai, Tamil Nadu, India for providing the radiation facilities necessary to carry out our research work. 42. Sergediene E, Jonsson K, Szymisiak H, Tyrakowsa B, Rietjens IM, Cenas N. Prooxidant toxicity of polyphenolic antioxidants to HL-60 cells description of quantitative structure activity relationships. FEBS Lett 1999; 462: 392-396.
doi:10.4149/bll_2016_033 fatcat:gg3rjudpqvftffpduhlo5f54wa