A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2018; you can also visit the original URL.
The file type is application/pdf
.
GlutathioneS-Transferase Pi Has at Least Three Distinguishable Xenobiotic Substrate Sites Close to Its Glutathione-binding Site
2004
Journal of Biological Chemistry
Benzyl isothiocyanate (BITC), present in cruciferous vegetables, is an efficient substrate of human glutathione S-transferase P1-1 (hGST P1-1). BITC also acts as an affinity label of hGST P1-1 in the absence of glutathione, yielding an enzyme inactive toward BITC as substrate. As monitored by using BITC as substrate, the dependence of k of inactivation (K I ) of hGST P1-1 on [BITC] is hyperbolic, with K I ؍ 66 ؎ 7 M. The enzyme incorporates 2 mol of BITC/mol of enzyme subunit upon complete
doi:10.1074/jbc.m407445200
pmid:15347687
fatcat:magmj6fxkre2vgklj2q4ede35y