Eichhornia crassipes is one of the aquatic plants that creates enormous annoyances because of its propensity to completely cover the surface of the water it colonizes, hence its negative impact on navigation, irrigation, fishing and the conservation of biodiversity. The aim of this work is its valorization by producing bioethanol of second generation; it is an optimal valorization for the production of simple fermentable sugars with high added value. In the first process which is chemical

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... ysis, the biomass was depolymerized by steam treatment. Then a second process which is enzymatic hydrolys is with Aspergillus Niger cellulase. The monitoring of kinetic parameters such as Brix, pH and glucose level showed a considerable evolution of the glucose level from 0.5 to 11.21 mg/ml according to the pretreatment time and from 11.21 to 21.46 mg/mL after the enzymatic hydrolysis according to the type of hydrolysed sample. In conclusion, this work allowed to show the impact of the thermomechanical and biotechnological processes used on the lignocellulosic biomass and to set up a time of pretreatment by steam explosion and an optimal concentration of cellulase applied to Eichhornia crassipes for its bioconversion in ethanol fuel.