Integrative Structure Modeling of Macromolecular Assemblies from Proteomics Data

Keren Lasker, Jeremy L. Phillips, Daniel Russel, Javier Velázquez-Muriel, Dina Schneidman-Duhovny, Elina Tjioe, Ben Webb, Avner Schlessinger, Andrej Sali
2010 Molecular & Cellular Proteomics  
Proteomics techniques have been used to generate comprehensive lists of protein interactions in a number of species. However, relatively little is known about how these interactions result in functional multiprotein complexes. This gap can be bridged by combining data from proteomics experiments with data from established structure determination techniques. Correspondingly, integrative computational methods are being developed to provide descriptions of protein complexes at varying levels of
more » ... uracy and resolution, ranging from complex compositions to detailed atomic structures. Molecular 1 The abbreviations used are: EM, electron microscopy; FRET, fluorescence resonance energy transfer; SAXS, small angle x-ray scattering; NPC, nuclear pore complex; AAA-ATPase, adenosine triphosphatase associated with diverse cellular activities; RNAPII, RNA polymerase II; Rpb, RNA polymerase II subunit; H-RNAPII, human RNA polymerase II; Y2H, yeast two-hybrid; RMSD, root mean square deviation. Review
doi:10.1074/mcp.r110.000067 pmid:20507923 pmcid:PMC2938050 fatcat:dzipnnyfqzf2dem67ulmkvdwjm