Imaging Spontaneous MMTVneu Transgenic Murine Mammary Tumors: Targeting Metabolic Activity Versus Genetic Products

M. L. Thakur, D. Devadhas, K. Zhang, R. G. Pestell, C. Wang, P. McCue, E. Wickstrom
2009 Journal of Nuclear Medicine  
Despite the great strides made in imaging breast cancer (BC) in humans, the current imaging modalities miss up to 30% of BC, do not distinguish malignant lesions from benign ones, and require histologic examinations for which invasive biopsy must be performed. Annually in the United States, approximately 5.6 million biopsies find benign lesions. More than 50% of human BCs overexpress cyclin D1, and all BCs exhibit VPAC1 oncogene products. Together, these gene products may provide an excellent
more » ... vide an excellent biomarker for the early and accurate detection of BC. We have evaluated 4 biologically active peptide analogs that have high affinity for VPAC1. The transgenic MMTVneu mice spontaneously develop BC and metastatic lesions that overexpress cyclin D1 and VPAC1 biomarkers. The MMTVneu mouse, therefore, provides an excellent animal model that mimics the pathogenesis of human BC. The objective of this investigation was to determine the ability of 1 of the peptide analogs, 64 Cu-TP3805, to detect BC in MMTVneu mice using 18 F-FDG as a gold standard. Methods: The transgenic MMTVneu mouse colony was maintained. Offspring were screened for transgenic status by reverse transcriptase polymerase chain reaction (RT-PCR). Nine mice with visible, palpable, or unknown metastatic lesions were entered into the protocol. 18 F-FDG (6,475 6 1,628 kBq [175 6 44 mCi]) PET served as a control, followed by a CT scan and 24-48 h later by PET with 64 Cu-TP3805 (4,588 6 962 kBq [124 6 26 mCi]). RT-PCR on excised tumors determined VPAC1 expression, and histology ascertained the pathology. Results: Ten tumors were detected by PET. Four tumors were detected both by 18 F-FDG and by 64 Cu-TP3805. Additionally, 4 tumors were imaged with 64 Cu-TP3805 only. These 8 tumors overexpressed VPAC1 receptors and were malignant by histology. The 2 remaining tumors were visualized with 18 F-FDG only. These tumors did not express the VPAC1 oncogene product and had benign histology. The standard uptake value ranged from 3.1 to 18.3 for 64 Cu-TP3805 and 0.9 to 1.4 for 18 F-FDG. Conclusion: 64 Cu-TP3805 identified all malignant lesions unequivocally that overexpressed the VPAC1 oncogene surface product. The 2 benign tumors that did not express the VPAC1 re-ceptor were not imaged. 64 Cu-TP3805 promises to have the potential for the early and accurate imaging of primary and metastatic BC.
doi:10.2967/jnumed.109.069542 pmid:20008985 fatcat:b3eved72cvha3cc7qogzjeeg6y