Prediction of Proteolytic Process Based on N-Terminal Sequences and Molecular Weights by Proteomics and Proteome Analysis

Yoshihumi Ujiie, Kouji Sasaki, Makoto Kinouchi, Yoshihiro Kudo, Shigehiko Kanaya
2000 Journal of Computer Aided Chemistry  
In proteomics and proteome analysis using 2-dimensional electrophoresis (2-DE), molecular weights and N-terminal sequences for individual protein spots are relatively easily determined. In the present study, we propose a procedure for estimating fragmentation process of a protein based on the inclusion relation among peptide sequence, and estimate fragmentation process of vitellogenin (Vtg) of Oncorhynchus masou based on N-terminal sequences and molecular weights for 48 fragments, that appear
more » ... ents, that appear at very high concentrations in the whole embryos of O. masou. A potential proteolytic process of Vtg is discussed in relation to normal and abnormal developmental embryos.
doi:10.2751/jcac.1.82 fatcat:6ksklgmmnjdh7bninzmzkgeav4