With the objective of developing new SSR markers for Coffea arabica, two enriched genomic libraries with probes (GT) 15 and (AGG) 10 were constructed. A total of 835 clones were sequenced and 756 presented good quality sequences. Redundant sequences were observed for 113 clones (14.94%). SSRs were found in 287 clones (38%). An estimated size of 417.5Kb of the C. arabica genome was sampled, with an average of one SSR per 1.46Kb. Dinucleotide repeats were more frequent than trinucleotides. Four

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... peat sequences, (AG/CT) n , (AC/GT) n , (AAG/CTT) n , and (AGG/CCT) n represented 61.1% of the total observed. A total of 96 SSR primers were designed and tested by PCR for two C. arabica genotypes. Ninety new SSR markers were validated for further genetic studies of C. arabica.