Objective-The metabolic syndrome predisposes to the development of cardiovascular diseases. Oxidative stress and elevated circulating oxidized low-density lipoprotein (LDL) concentrations are related to cardiovascular disease and proposed to be features of the metabolic syndrome. F 2 -isoprostanes are lipid peroxidation products and considered the most reliable biomarkers of oxidative stress. Methods and Results-Plasma oxidized LDL (oxLDL) and urinary 8-iso-prostaglandin F 2␣ (8-iso-PGF 2␣ ;

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... major F 2 -isoprostane) were analyzed in a cross-sectional study of 289 healthy men (62 to 64 years of age). Individuals completed a 7-day dietary record, and fasting plasma insulin, lipid, and lipoprotein concentrations, LDL particle size, and inflammatory markers were determined. National Cholesterol Education Program/Adult Treatment Panel III (NCEP/ATPIII) criteria were used to define the metabolic syndrome and individuals were grouped according to the number of risk factors for the metabolic syndrome (0, [nϭ88; 30%]; Ն1, [nϭ179; 62%], metabolic syndrome [nϭ22; 8%]). Group comparisons revealed no differences for oxLDL, 8-iso-PGF 2␣ , or reported intake of macronutrients, whereas C-reactive protein and interleukin-6 were increased in the metabolic syndrome. LDL cholesterol strongly determined oxLDL in univariate and multivariate analysis, but no relationship to 8-iso-PGF 2␣ was found. In turn, 8-iso-PGF 2␣ was related to reported intake of fat, fatty acids, and dietary antioxidants. Conclusions-There were no increases in plasma oxLDL or measures of oxidative stress (urinary 8-iso-PGF 2␣ ) in these otherwise healthy 63-year-old men with the metabolic syndrome. Furthermore, no relationship between oxLDL and 8-iso-PGF 2␣ was found, but our results suggest a role for dietary factors in oxidative stress. (Arterioscler Thromb Vasc Biol. 2005;25:2580-2586.)