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Methods in Enzymology
Two-hybrid systems are one of the most popular, preferred, cost effective, and scalable in vivo genetic approaches for screening protein-protein interactions. A number of variants of yeast and bacterial two-hybrid systems exist, rendering them ideal for modern, flexible proteomics-driven studies. For mapping protein interactions at genome scales (that is, constructing an interactome), the yeast two-hybrid system has been extensively tested and is preferred over bacterial two-hybrid systems,doi:10.1016/bs.mie.2016.10.020 pmid:28137570 pmcid:PMC5737774 fatcat:vrsskowwrva47lef4xlqtld7si