An invasive neoplasm from embryonic cells of Drosophila melanogaster cultured permanently in vivo (author's transl)
Ein invasives Neoplasma aus embryonalen Zellen von Drosophila melanogaster in Dauerkultur in vivo

H P Hauri
1974 Journal of embryology and experimental morphology  
An invasive neoplasm from embryonic cells of Drosophila melanogaster cultured permanently in vivo The neoplasm H 126, obtained from the posterior half of a 6 + 1-h-old embryo of Drosophila melanogaster, was cultivated in adult female flies over more than 120 transfer generations (more than 4 years). Four sub-lines were derived from it. The neoplasm H 126 invades the ovaries of the host flies, whereas the intestinal tract and the fat body are only surrounded very tightly, but not invaded by
more » ... not invaded by these abnormal cells. The neoplasm is lethal to the host 8-14 days after implantation into the abdomen (cultivation temperature: 25 °C). Egg deposition of neoplasm-bearing hosts decreases 5 days after abdominal implantation. After 4 years of cultivation the sub-lines differ specifically from one another in their chromosomal content; however, all of them show a karyotype near tetraploidy. Ultrastructural features of these neoplasm cells are: (a) the nearly complete absence of membrane specializations correlated with weak cell adhesion, (b) the abundance of cytoplasmic processes and (c) 36 nm virus-like particles in the nucleus and in the cytoplasm. Invasiveness, alterations of the karyotype, the origin of the neoplasm HI26, and the presence of virus-like particles are discussed.
pmid:4211294 fatcat:wjs5ubwl5jc5zojiebfcwqmjwi