High performance crystalline nanocellulose using an ancestral endoglucanase

Borja Alonso-Lerma, Leire Barandiaran, Lorena Ugarte, Izaskun Larraza, Antonio Reifs, Raquel Olmos-Juste, Nerea Barruetabeña, Iban Amenabar, Rainer Hillenbrand, Arantxa Eceiza, Raul Perez-Jimenez
2020 Communications Materials  
Cellulose fiber Macrofiber Microfiber Endoglucanase CBM Cellulose nanocrystal CNC Elementary fibrils Nanocellulose water suspension Fig. 1 Illustration of cellulose enzymatic hydrolysis. The hierarchical structure of cellulose fiber permits its degradation into nano scale particles. Enzymatic hydrolysis was performed in water at 50°C at different times in agitation with different endoglucanase. Here, we show how endoglucanase with CBM can attach to the cellulose fiber placing the catalytic
more » ... the catalytic domain in the fiber surface and improving its activity. Enzymes degradation can produce nanocellulose with different sizes depending on the hydrolysis time and enzyme usage. Cellulose nanocrystals (CNC) are small crystalline particles with lengths between 100 and 500 nm and diameters between 3 and 40 nm. ARTICLE COMMUNICATIONS MATERIALS | https://doi.
doi:10.1038/s43246-020-00055-5 fatcat:thnkjazix5aubfmw66iw4uauli