Quantitative analysis of Acyclovir in pure form and pharmaceutical preparations

Abd Alhamide
2014 International Journal of Chemical and Pharmaceutical Sciences   unpublished
Three spectrophotometric methods are described for quantitative analysis of acyclovir in pure and dosage forms. The first method was depended on insist generated bromine as oxidizing agent and either methyl orange or methyl blue as a chromogenic agents acyclovir was treated with known excess amounts of bromine and residual unreacted bromine is determined by treating with fixed amount of either methyl orange and measure absorbance at 550 mm or methyl blue and measure absorbance at 650 nm. The
more » ... e at 650 nm. The amount of bromine reacts equal to acyclovir. Calibration curves were cover over ranges (1-30) µg/ml, for methyl orange and (2.5-40) µg/ml. The second method was depended on diazo coupling of acyclovir with resorcinol to form yellow-orange color with maximum absorption at 462 µm, Beer's law was obeyed in the range of (1-40) µg/ml. The third method was depended on the formation of yellow Schiff base complex as a result of condensation reaction between cited drug and 2.4-dihydroxy benzaldehyde in ethanol solution which absorbed maximally at 435nm with abeyance with Beers law in the concentration range of (5-100) µg/ml, the methods were satisfactory applied for determination of acyclovir in pure and dosage forms and results were compared statistically with reference method (HPLC).