Predominant Expression of Platelet-Activating Factor Receptor in the Rat Brain Microglia

Mikiro Mori, Makoto Aihara, Kazuhiko Kume, Makoto Hamanoue, Shinichi Kohsaka, Takao Shimizu
1996 Journal of Neuroscience  
Cellular localization of platelet-activating factor (PAF) receptor in the rat brain was determined by (1) in situ hybridization, (2) Northern blot analysis in primary cell cultures of neurons, microglia, astrocytes, and fibroblasts, and (3) Ca2+ imaging in hippocampal culture. In situ hybridization revealed that the PAF receptor mRNA is expressed intensely in microglia and moderately in neurons. Northern blot analysis revealed that PAF receptor mRNA is the most abundant in microglia. In primary
more » ... croglia. In primary hippocampal cultures, PAF elevated intracellular Ca*+ concentration in microglia and also in neurons, but to a lesser extent. These results suggest predominant presence of PAF receptor in microglia. Cultured microglia also expressed cPLA, mRNA the most intensely. PAF-activated microglia released arachidonic acid in a Ca*+-dependent manner and without conversion to its derivatives. We propose that microglia as well as neurons contribute to PAF-related events in the CNS by releasing arachidonic acid.
doi:10.1523/jneurosci.16-11-03590.1996 pmid:8642404 fatcat:xc6gvlkeangpharuvuzlkdvku4