Expression of MUC5B Protein and mRNA in Gallbladder Membrane Directly Correlated with Gallstone Nucleation

Sanjeev Kumar Maurya
2014 Anatomy & Physiology  
The presence of gallstones in the gallbladder (Cholecystolithiasis) is a common disease in Northern part of India and some other countries like japan, China and Indo-American population. Nucleation is first event for the formation of gallstone in gallbladder sac environment. It was suspected that mucin family gene play role in nucleation process. Out of 20 mucin family members it is still unclear which one playing crucial role in gallstone nucleation process. Our objective was to investigate
more » ... retary/jell forming mucins in gallstone formation. Mucin family genes [MUC1, MUC5AC, MUC5B, MUC6, MUC7, MUC9 and MUC11] were evaluated through western blotting and semiquantitative PCR. MUC5B mRNA level was most prominent in expression followed by MUC9 and MUC5AC. MUC5B expression was much significantly higher in cancerous gallbladder than non-cancerous gallbladder (p<0.001, CI95%). MUC5B expression was directly correlated with number of stone. Whereas size of stone was not having any impact on MUC5B upregulation. Above result indicate that MUC5B protein may crucially involve in nucleation process during gallstone formation. Citation: Maurya SK, Tewari M, Khan AA, Shukla HS (2014) Expression of MUC5B Protein and mRNA in Gallbladder Membrane Directly Correlated with Gallstone Nucleation . Anat Physiol 4: 155.
doi:10.4172/2161-0940.1000155 fatcat:sbfizm75nbbxpcq7v7h3fqqbse