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Mass allelic exchange: enabling sexual genetics in Escherichia coli
Despite dramatic advances in genomics, connecting genotypes to phenotypes is still challenging. Sexual genetics combined with linkage analysis is a powerful solution to this problem but generally unavailable in bacteria. We build upon a strong negative selection system to invent Mass Allelic Exchange (MAE), which enables hybridization of arbitrary (including pathogenic) strains of E. coli. MAE reimplements the natural phenomenon of random crossovers, enabling classical linkage analysis. Wedoi:10.1101/2020.11.30.405282 fatcat:ss3bjseqtnaufkowibaeanxhvi