A copy of this work was available on the public web and has been preserved in the Wayback Machine. The capture dates from 2017; you can also visit the original URL.
The file type is application/pdf
.
Capillary isoelectric focusing of a difficult-to-denature tetrameric enzyme using alkylurea–urea mixtures
2014
Analytical Biochemistry
Alkylurea Capillary isoelectric focusing Capillary electrophoresis a b s t r a c t Capillary isoelectric focusing (cIEF) is normally run under denaturing conditions using urea to expose any buried protein residues that may contribute to the overall charge. However, urea does not completely denature some proteins, such as the tetrameric enzyme Erwinia chrysanthemi L-asparaginase (ErA), in which case electrophoresis-compatible alternative denaturants are required. Here, we show that alkylureas
doi:10.1016/j.ab.2014.08.004
pmid:25132564
fatcat:m3dnholvg5fjbdwakl5rry66ii