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Capillary isoelectric focusing of a difficult-to-denature tetrameric enzyme using alkylurea–urea mixtures
Alkylurea Capillary isoelectric focusing Capillary electrophoresis a b s t r a c t Capillary isoelectric focusing (cIEF) is normally run under denaturing conditions using urea to expose any buried protein residues that may contribute to the overall charge. However, urea does not completely denature some proteins, such as the tetrameric enzyme Erwinia chrysanthemi L-asparaginase (ErA), in which case electrophoresis-compatible alternative denaturants are required. Here, we show that alkylureasdoi:10.1016/j.ab.2014.08.004 pmid:25132564 fatcat:m3dnholvg5fjbdwakl5rry66ii