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Methanol was examined as a cryoprotective additive that permits the direct transfer of frozen\p=n-\thawed Day-4 mouse embryos to foster mothers without dilution of the cryoprotectant. Methanol permeated the embryos rapidly, was not toxic and exerted a cryoprotective action. The highest level of survival (50%) of embryos in vitro was observed after equilibration in Medium PB1 containing 3\m=.\0 M-methanol, slow cooling (0\m=.\5\s=deg\C/mi n) to a temperature between \m=-\30 and \doi:10.1530/jrf.0.0700293 pmid:6363691 fatcat:omnjvea6grgklgznhdzzqim7la