TheH19Transcript Is Associated with Polysomes and May RegulateIGF2Expression intrans

Yi-Ming Li, Gary Franklin, Heng-Mi Cui, Kristian Svensson, Xiao-Bing He, Gail Adam, Rolf Ohlsson, Susan Pfeifer
1998 Journal of Biological Chemistry  
The imprinted H19 gene produces a fully processed transcript that does not exhibit any conserved open reading frame between mouse and man. Although transcriptional control elements associated with the mouse H19 locus have been shown to control the neighboring Igf2 gene in cis, the prevailing view is that the cytoplasmic H19 transcript does not display any function. In contrast to earlier reports, we show here that the H19 transcript is associated with polysomes in a variety of cell types, in
more » ... f cell types, in both mouse and man. A possible transfunction of the H19 gene is suggested by a reciprocal correlation in trans between cytoplasmic H19 and IGF2 mRNA levels, as well as IGF2 mRNA translatability. We discuss these results in terms of their challenge to the prevailing dogma on the function of the enigmatic H19 gene, as well as with respect to the ontogeny of the Beckwith-Wiedemann syndrome, and propose that the human H19 gene is an antagonist of IGF2 expressivity in trans.
doi:10.1074/jbc.273.43.28247 pmid:9774446 fatcat:eevtaputbfadrldlx4ovxdcz2u