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Glucanase (endo-beta-1, 3-glucan 3-glucanohydrolase, EC 18.104.22.168, laminarinase, callase) and chitinase (poly-beta-1, 4-[2-acetamido-2-deoxy] -d-glucoside glycanohydrolase, EC 22.214.171.124) were extracted from ethylene-treated bean (Phaseolus vulgaris L. cv. Red Kidney) leaves and purified on hydroxyapatite and carboxymethyl Sephadex columns. The glucanase prepared was homogeneous as judged by analytical centrifugation data, electrophoresis, and antibody-antigen reactions. On the basis of geldoi:10.1104/pp.47.1.129 pmid:16657567 pmcid:PMC365825 fatcat:f6gkczrlibdclkisfkiehwvx5u