Multilayer based microfluidic cell culture systems with integrated actuators for animal free substance testing

Florian Schmieder, Stefan Grünzner, Joachim Ströbel, Mathias Busek, Frank Sonntag
2015
Schematic exploded view of a multilayer based microfluidic system with several micropumps and valves; the function of the different layers is discribed in the table below FIGURE 2: Left: microfluidic channel endothelialized with HUVEC after one day under perfusion; the main image shows a phase contrast picture of the endothelial cells; the detail shows the same cells stained with CellTracker™ Red CMTPX; right: fluorescent double staining oft HUVEC under perfusion; blue: DAPI-staining of the
more » ... eus; red: fluorescence based staining of van Willebrandfactor with Alexa Fluor® 633; white arrows indicate the direction of flow
doi:10.24406/publica-fhg-390308 fatcat:lcofeyjaizgmra4rmiqoaw4cnq